Nanopore Filter: A Method for Counting and Extracting Single DNA Molecules Using a Biological Nanopore.

ABSTRACT: This paper describes a method for the real-time counting and extraction of DNA molecules at the single-molecule level by nanopore technology. As a powerful tool for electrochemical single-molecule detection, nanopore technology eliminates the need for labeling or partitioning sample solutions at the femtoliter level. Here, we attempt to develop a DNA filtering system utilizing an α-hemolysin (αHL) nanopore. This system comprises two droplets, one filling with and one emptying DNA molecules, separated by a planar lipid bilayer containing αHL nanopores. The translocation of DNA through the nanopores is observed by measuring the channel current, and the number of translocated molecules can also be verified by quantitative polymerase chain reaction (qPCR). However, we found that the issue of c

SUBMITTER: Tada A 

PROVIDER: S-EPMC10797584 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature