The functional role of CST1 and CCL26 in asthma development.

ABSTRACT:

Background

Asthma is the most common chronic disease in children with an increasing prevalence. Its development is caused by genetic and environmental factors and allergic sensitization is a known trigger. Dog allergens affect up to 30% of all children and dog dander-sensitized children show increased expression of cystatin-1 (CST1) and eotaxin-3 (CCL26) in nasal epithelium. The aim of our study was to investigate the functional mechanism of CST1 and CCL26 in the alveolar basal epithelial cell line A549.

Methods

A549 cells were transfected with individual overexpression vectors for CST1 and CCL26 and RNA sequencing was performed to examine the transcriptomics. edgeR was used to identify differentially expressed genes (= DEG, |log₂ FC | ≥ 2, FDR < 0.01). The protei

SUBMITTER: Hoyer A 

PROVIDER: S-EPMC10797655 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature