Evaluation of novel chromatographic prototypes for supercoiled plasmid DNA polishing.

ABSTRACT: Since the world first approved gene therapeutics, nucleic acid-based therapies have gained prominence. Several strategies for DNA-based therapy have been approved, and numerous clinical trials for plasmid DNA (pDNA)-based vaccines are currently in development. Due to the rising interest in pDNA for vaccination and gene therapy, plasmid manufacturing must become more effective. One of the most critical steps is downstream processing, involving isolation and purification procedures. To comply with the regulatory guidelines, pDNA must be available as a highly purified, homogeneous preparation of supercoiled pDNA (sc pDNA). This process undertakes several challenges, primarily due to the diversity of molecules derived from the producer organism. In this study, different resins were tested for

SUBMITTER: Ferreira PL 

PROVIDER: S-EPMC10797707 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature