Global transcriptome analysis of the C57BL/6J mouse testis by SAGE: evidence for nonrandom gene order.

ABSTRACT:

Background

We generated the gene expression profile of the total testis from the adult C57BL/6J male mice using serial analysis of gene expression (SAGE). Two high-quality SAGE libraries containing a total of 76 854 tags were constructed. An extensive bioinformatic analysis and comparison of SAGE transcriptomes of the total testis, testicular somatic cells and other mouse tissues was performed and the theory of male-biased gene accumulation on the X chromosome was tested.

Results

We sorted out 829 genes predominantly expressed from the germinal part and 944 genes from the somatic part of the testis. The genes preferentially and specifically expressed in total testis and testicular somatic cells were identified by comparing the testis SAGE transcriptomes to the available tran

SUBMITTER: Divina P 

PROVIDER: S-EPMC1079818 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature