Conversion of cDNA differential display results (DDRT-PCR) into quantitative transcription profiles.

ABSTRACT:

Background

Gene expression studies on non-model organisms require open-end strategies for transcription profiling. Gel-based analysis of cDNA fragments allows to detect alterations in gene expression for genes which have neither been sequenced yet nor are available in cDNA libraries. Commonly used protocols for gel-based transcript profiling are cDNA differential display (DDRT-PCR) and cDNA-AFLP. Both methods have been used merely as qualitative gene discovery tools so far.

Results

We developed procedures for the conversion of cDNA Differential Display data into quantitative transcription profiles. Amplified cDNA fragments are separated on a DNA sequencer and detector signals are converted into virtual gel images suitable for semi-automatic analysis. Data processing consists

SUBMITTER: Venkatesh B 

PROVIDER: S-EPMC1079827 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature