Heterologous expression in Tritrichomonas foetus of functional Trichomonas vaginalis AP65 adhesin.

ABSTRACT:

Background

Trichomonosis, caused by Trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. The interaction of T. vaginalis with vaginal epithelial cells (VECs), a step preparatory to infection, is mediated in part by the prominent surface protein AP65. The bovine trichomonad, Tritrichomonas foetus, adheres poorly to human VECs. Thus, we established a transfection system for heterologous expression of the T. vaginalis AP65 in T. foetus, as an alternative approach to confirm adhesin function for this virulence factor.

Results

In this study, we show stable transfection and expression of the T. vaginalis ap65 gene in T. foetus from an episomal pBS-ap65-neo plasmid. Expression of the ge

SUBMITTER: Kucknoor AS 

PROVIDER: S-EPMC1079839 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature