Feasibility demonstration of a device for vitreous liquid biopsy incidental to intravitreal injection.

ABSTRACT:

Purpose

VitreoDx is an experimental device enabling push-button collection of a neat vitreous liquid biopsy incidental to an intravitreal injection. We explored the ability of the device to collect a sample usable for proteomic biomarker discovery and testing.

Design

Pilot study using ex vivo human eyes.

Methods

Non-vitrectomized, human eyes from nine donors 75-91 years of age were refrigerated in BSS and used within 5 days of death. Four VitreoDx devices fitted with 25G needles, and four staked needle insulin syringes with 30G needles, were inserted at equal intervals through the pars plana of each eye and held in place by a fixture. The sampling mode of each VitreoDx device was triggered to attempt to acquire a liquid biopsy up to 70 μL. The plunger of each insulin

SUBMITTER: Tumlinson AR 

PROVIDER: S-EPMC10798501 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature