Towards high-throughput parallel imaging and single-cell transcriptomics of microbial eukaryotic plankton.

ABSTRACT: Single-cell transcriptomics has the potential to provide novel insights into poorly studied microbial eukaryotes. Although several such technologies are available and benchmarked on mammalian cells, few have been tested on protists. Here, we applied a microarray single-cell sequencing (MASC-seq) technology, that generates microscope images of cells in parallel with capturing their transcriptomes, on three species representing important plankton groups with different cell structures; the ciliate Tetrahymena thermophila, the diatom Phaeodactylum tricornutum, and the dinoflagellate Heterocapsa sp. Both the cell fixation and permeabilization steps were adjusted. For the ciliate and dinoflagellate, the number of transcripts of microarray spots with single cells were significantly higher than fo

SUBMITTER: Grujcic V 

PROVIDER: S-EPMC10798536 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature