Baseline azithromycin resistance in the gut microbiota of preterm born infants.

ABSTRACT:

Background

Macrolides, including azithromycin, are increasingly used in preterm-born infants to treat Ureaplasma infections. The baseline carriage of macrolide resistance genes in the preterm stool microbiota is unknown.

Objectives

Identify carriage of azithromycin resistant bacteria and the incidence of macrolide resistant genes.

Methods

Azithromycin resistant bacteria were isolated from serial stool samples obtained from preterm infants (≤32 weeks' gestation) by culturing aerobically/anaerobically, in the presence/absence of azithromycin. Using quantitative PCR, we targeted 6 common macrolide resistance genes (erm(A), erm(B), erm(C), erm(F), mef(A/E), msr(A)) in DNA extracted from selected bacteria resistant to azithromycin.

Results

From 89 stool samples from

SUBMITTER: Gallacher DJ 

PROVIDER: S-EPMC10798878 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature