A novel Escherichia coli cell-based bioreporter for quantification of salicylic acid in cosmetics.

ABSTRACT: Transcription factor-based bioreporters have been extensively studied for monitoring and detecting environmental toxicants. In Escherichia coli, the multiple antibiotic resistance regulator (MarR) induces transcription upon binding to salicylic acid (SA). We generated SA-specific E. coli cell-based bioreporters utilizing the operator region of the mar operon and MarR as components of the reporter and sensing domains, respectively. Although bioreporters based on endogenous MarR and wild-type E. coli cells responded to SA, their sensitivity and selectivity were insufficient for practical sample monitoring. To improve these parameters, we genetically engineered host strains for optimal MarR expression, which enhanced the sensitivity of the biosensor to micromolar quantities of SA with increas

SUBMITTER: Kim Y 

PROVIDER: S-EPMC10799119 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature