New biomarkers underlying acetic acid tolerance in the probiotic yeast Saccharomyces cerevisiae var. boulardii.

ABSTRACT: Evolutionary engineering experiments, in combination with omics technologies, revealed genetic markers underpinning the molecular mechanisms behind acetic acid stress tolerance in the probiotic yeast Saccharomyces cerevisiae var. boulardii. Here, compared to the ancestral Ent strain, evolved yeast strains could quickly adapt to high acetic acid levels (7 g/L) and displayed a shorter lag phase of growth. Bioinformatic-aided whole-genome sequencing identified genetic changes associated with enhanced strain robustness to acetic acid: a duplicated sequence in the essential endocytotic PAN1 gene, mutations in a cell wall mannoprotein (dan4^Thr192del), a lipid and fatty acid transcription factor (oaf1^Ser57Pro) and a thiamine biosynthetic enzyme (thi13^Thr332Ala). I

SUBMITTER: Samakkarn W 

PROVIDER: S-EPMC10799125 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature