A flexible open-source processing workflow for multiplexed fluorescence imaging based on cycles.

ABSTRACT: Background  Multiplexing tissue imaging is developing as a complement for single cell analysis, bringing the spatial information of cells in tissue in addition to multiple parameters measurements. More and more commercial or home-made systems are available. These techniques allow the imaging of tens of fluorescent reporters, where the spectral overlap is solved by imaging by cycles the fluorophores using microfluidics to change the reporters between each cycle. Methods  For several systems, the acquisition system coupled to the microfluidic system is a wide field microscope, and the acquisition process is done by mosaicking to cover a large field of view, relying on image processing to obtain the data set to be analysed in intensity. The processed data set allows the identifi

SUBMITTER: Potier G 

PROVIDER: S-EPMC10799232 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature