Baicalin enhances proliferation and reduces inflammatory-oxidative stress effect in H₂O₂-induced granulosa cells apoptosis via USP48 protein regulation.

ABSTRACT:

Background

Oxidative stress and inflammation can lead to apoptosis of ovarian granulosa cells (GCs), resulting in ovulation disorders and infertility. Baicalin (BAI) promotes cell proliferation and reduces inflammation and oxidative stress. However, the mechanisms by which BAI treatment affects oxidative stress and inflammation in GCs remain incompletely understood.

Methods

KGN cells were treated with hydrogen peroxide (H₂O₂) to analyze the effect of oxidative stress on GCs in vitro. Subsequently, H₂O₂-stimulated KGN cells were treated with BAI. The levels of GSH-Px, CAT, and SOD were measured using an activity assay kit. The levels of MDA, IL-1β, IL-6, IL-8, and TNF-α were measured by ELISA. Proliferation, apoptosis, and mRNA and

SUBMITTER: Chen J 

PROVIDER: S-EPMC10799411 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature