Highly selective whole-cell 25-hydroxyvitamin D₃ synthesis using molybdenum-dependent C25-steroid dehydrogenase and cyclodextrin recycling.

ABSTRACT:

Background

The global prevalence of vitamin D (VitD) deficiency associated with numerous acute and chronic diseases has led to strategies to improve the VitD status through dietary intake of VitD-fortified foods and VitD supplementation. In this context, the circulating form of VitD₃ (cholecalciferol) in the human body, 25-hydroxy-VitD₃ (calcifediol, 25OHVitD₃), has a much higher efficacy in improving the VitD status, which has motivated researchers to develop methods for its effective and sustainable synthesis. Conventional monooxygenase-/peroxygenase-based biocatalytic platforms for the conversion of VitD₃ to value-added 25OHVitD₃ are generally limited by a low selectivity and yield, costly reliance on cyclodextrins and elect

SUBMITTER: Kosian D 

PROVIDER: S-EPMC10799449 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature