Methionine enkephalin (MENK) protected macrophages from ferroptosis by downregulating HMOX1 and ferritin.

ABSTRACT:

Objective

The aim of this work was to investigate the immunological effect of MENK by analyzing the protein spectrum and bioinformatics of macrophage RAW264.7, and to explore the relationship between macrophage and ferroptosis.

Result

We employed proteomic analysis to identify differentially expressed proteins (DEPs) between macrophages and macrophages intervened by MENK. A total of 208 DEPs were identified. Among these, 96 proteins had upregulated expression and 112 proteins had downregulated expression. Proteomic analysis revealed a significant enrichment of DEPs associated with iron metabolism. The identification of hub genes was conducted using KEGG pathway diagrams and protein-protein interaction (PPI) analysis. The hub genes identified in this study include HMOX1 and F

SUBMITTER: Tian J 

PROVIDER: S-EPMC10799539 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature