Two dimensional VOPBA reveals laminin receptor (LAMR1) interaction with dengue virus serotypes 1, 2 and 3.

ABSTRACT:

Background

The search for the dengue virus receptor has generated many candidates often identified only by molecular mass. The wide host range of the viruses in vitro combined with multiple approaches to identifying the receptor(s) has led to the notion that many receptors or attachment proteins may be involved and that the different dengue virus serotypes may utilize different receptors on the same cells as well as on different cell types.

Results

In this study we used sequential extraction of PS Clone D cell monolayers with the detergent beta-octylglucopyranoside followed by sodium deoxycholate to prepare a cell membrane-rich fraction. We then used 2 dimensional (2D) gel electrophoresis to separate the membrane proteins and applied a modified virus overlay protein binding

SUBMITTER: Tio PH 

PROVIDER: S-EPMC1079963 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature