The naringenin-dependent regulator FdeR can be applied as a NIMPLY gate controlled by naringenin and arabinose.

ABSTRACT: The FdeR regulator has been reported as a transcriptional activator dependent on the interaction with naringenin. Previously, FdeR and its cognate promoter were used to construct naringenin-sensitive sensors, though no correlation was associated between the FdeR level of expression and outputs. Therefore, to understand this correlation, we constructed a circuit with FdeR expression adjusted by the arabinose concentration through an AraC-P_BAD system and the FdeR-regulated promoter controlling the expression of GFP. We observed a significant reduction in the activity of the target promoter by increasing FdeR expression, indicating that although FdeR has been primarily classified as a transcriptional activator, it also represses transcription. Leveraging the bifunctional fea

SUBMITTER: Kashiwagi FM 

PROVIDER: S-EPMC10799723 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature