Glutathione Transferase Photoaffinity Labeling Displays GST Induction by Safeners and Pathogen Infection.

ABSTRACT: Glutathione transferases (GSTs) represent a large and diverse enzyme family involved in the detoxification of small molecules by glutathione conjugation in crops, weeds and model plants. In this study, we introduce an easy and quick assay for photoaffinity labeling of GSTs to study GSTs globally in various plant species. The small-molecule probe contains glutathione, a photoreactive group and a minitag for coupling to reporter tags via click chemistry. Under UV irradiation, this probe quickly and robustly labels GSTs in crude protein extracts of different plant species. Purification and mass spectrometry (MS) analysis of labeled proteins from Arabidopsis identified 10 enriched GSTs from the Phi(F) and Tau(U) classes. Photoaffinity labeling of GSTs demonstrated GST induction in wheat seedli

SUBMITTER: Font Farre M 

PROVIDER: S-EPMC10799724 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature