Immunoreactivity of canine, feline, and equine D-dimer with antibodies to human D-dimer.

ABSTRACT:

Background

Commercially available D-dimer assays use antibodies against human D-dimer, with limited sensitivity and specificity data in companion animals.

Objectives

To evaluate the immunoreactivity of D-dimer in plasma of dogs, horses, and cats with commercially available antibodies to human D-dimer.

Animals

Plasma samples were collected from healthy dogs and horses, and from surplus feline plasma submitted for diagnostic purposes.

Methods

Descriptive research study. A cross-linked fibrin lysate was prepared from plasma samples, and SDS-PAGE and immunoblotting were performed with a variety of commercially available antibodies to human D-dimer.

Results

The selected antibodies demonstrated variable reactivity with D-dimer of each species. The monoclonal a

SUBMITTER: Brown JE 

PROVIDER: S-EPMC10800179 | biostudies-literature | 2024 Jan-Feb

REPOSITORIES: biostudies-literature