Rapid characterization of adeno-associated virus (AAV) capsid proteins using microchip ZipChip CE-MS.

ABSTRACT: Adeno-associated viruses (AAVs) are viral vectors used as delivery systems for gene therapies. Intact protein characterization of AAV viral capsid proteins (VPs) and their post-translational modifications is critical to ensuring product quality. In this study, microchip-based ZipChip capillary electrophoresis-mass spectrometry (CE-MS) was applied for the rapid characterization of AAV intact VPs, specifically full and empty viral capsids of serotypes AAV6, AAV8 and AAV9, which was accomplished using 5 min of analysis time. Low levels of dimethyl sulfoxide (4%) in the background electrolyte (BGE) improved MS signal quality and component detection. A sensitivity evaluation revealed consistent detection of VP proteoforms when as little as 2.64 × 10⁶ viral particles (≈26.4 picograms)

SUBMITTER: Smith J 

PROVIDER: S-EPMC10800304 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature