Multifactorial analysis of terminator performance on heterologous gene expression in Physcomitrella.

ABSTRACT:

Key message

Characterization of Physcomitrella 3'UTRs across different promoters yields endogenous single and double terminators for usage in molecular pharming. The production of recombinant proteins for health applications accounts for a large share of the biopharmaceutical market. While many drugs are produced in microbial and mammalian systems, plants gain more attention as expression hosts to produce eukaryotic proteins. In particular, the good manufacturing practice (GMP)-compliant moss Physcomitrella (Physcomitrium patens) has outstanding features, such as excellent genetic amenability, reproducible bioreactor cultivation, and humanized protein glycosylation patterns. In this study, we selected and characterized novel terminators for their effects on heterologous gene expres

SUBMITTER: Niederau PA 

PROVIDER: S-EPMC10800305 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature