Influence of the mRNA initial region on protein production: a case study using recombinant detoxified pneumolysin as a model.

ABSTRACT: Recombinant proteins are of great importance in modern society, mostly as biopharmaceutical products. However, challenging and complex processes with low production yield are major drawbacks. Normally, the optimization to overcome these obstacles is focused on bioreactor and purification processes, and the biomolecular aspects are neglected, seen as less important. In this work, we present how the 5' mRNA secondary structure region can be relevant for translation and, therefore, protein production. For this, Escherichia coli BL21(DE3) clones, producing recombinant detoxified pneumolysin (PdT) with and without the N-terminal His-tag, were cultivated in 10-L bioreactors. Another version of the pdt gene (version 2) with synonymous changes in the 5'-end nucleotide sequence was al

SUBMITTER: Fusco F 

PROVIDER: S-EPMC10800503 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature