Observing exocrine pancreas metabolism using a novel pancreas perfusion technique in combination with hyperpolarized [1-¹³ C]pyruvate.

ABSTRACT: In a clinical setting, ex vivo perfusions are routinely used to maintain and assess organ viability prior to transplants. Organ perfusions are also a model system to examine metabolic flux while retaining the local physiological structure, with significant success using hyperpolarized (HP) ¹³ C NMR in this context. We use a novel exocrine pancreas perfusion technique via the common bile duct to assess acinar cell metabolism with HP [1-¹³ C]pyruvate. The exocrine component of the pancreas produces digestive enzymes through the ductal system and is often neglected in research on the pancreas. Real-time production of [1-¹³ C]lactate, [1-¹³ C]alanine, [1-¹³ C]malate, [4-¹³ C]malate, [1-¹³ C]aspartate, and H¹³

SUBMITTER: Rushin A 

PROVIDER: S-EPMC10800648 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature