Specific changes in amino acid profiles in monocytes of patients with breast, lung, colorectal and ovarian cancers.

ABSTRACT:

Introduction

Immunometabolism is essential factor of tumor progression, and tumor-associated macrophages are characterized by substantial changes in their metabolic status. In this study for the first time, we applied targeted amino acid LC-MS/MS analysis to compare amino acid metabolism of circulating monocytes isolated from patients with breast, ovarian, lung, and colorectal cancer.

Methods

Monocyte metabolomics was analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/ MS) analysis of amino acid extracts. The targeted analysis of 26 amino acids was conducted by LCMS/MS on an Agilent 6460 triple quadrupole mass spectrometer equipped with an electrospray ionization source and an Agilent 1260 II liquid chromatograph.

Results

Comparison of monocy

SUBMITTER: Chagovets V 

PROVIDER: S-EPMC10800720 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature