Optimized recombinant production of the bacteriocin garvicin Q by Corynebacterium glutamicum.

ABSTRACT: Bacteriocins are antimicrobial peptides applied in food preservation and are interesting candidates as alternatives to conventional antibiotics or as microbiome modulators. Recently, we established Corynebacterium glutamicum as a suitable production host for various bacteriocins including garvicin Q (GarQ). Here, we establish secretion of GarQ by C. glutamicum via the Sec translocon achieving GarQ titers of about 7 mg L^-1 in initial fermentations. At neutral pH, the cationic peptide is efficiently adsorbed to the negatively charged envelope of producer bacteria limiting availability of the bacteriocin in culture supernatants. A combination of CaCl₂ and Tween 80 efficiently reduces GarQ adsorption to C. glutamicum. Moreover, cultivation in minimal

SUBMITTER: Desiderato CK 

PROVIDER: S-EPMC10800739 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature