Development and validation of a novel detection method for Rickettsia rickettsii using a loop-mediated isothermal amplification assay.

ABSTRACT:

Introduction

Rickettsia rickettsii is an obligate, intracellular pathogen and the causative agent of Rocky Mountain spotted fever (RMSF). RMSF is an important zoonotic disease due to its high fatal outcome in humans. The difficulty of clinical diagnosis due to the low sensitivity and specificity of current diagnostic methods are a principal setback. We reported the development of a new method for the detection of R. rickettsii in human and tick DNA samples using loop-mediated isothermal amplification (LAMP), as well as the validation of the LAMP test for R. rickettsii in field samples of infected ticks and humans, determining the diagnostic sensitivity and specificity, as well as the reproducibility of the test.

Methods

This technique uses hydroxy naphth

SUBMITTER: Carvajal-Gamez BI 

PROVIDER: S-EPMC10800886 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature