Optimized protocol for quantification of extracellular nicotinamide adenine dinucleotide: evaluating clinical parameters and pre-analytical factors for translational research.

ABSTRACT: Nicotinamide adenine dinucleotide (NAD⁺), a coenzyme for more than 500 enzymes, plays a central role in energy production, metabolism, cellular signaling, and DNA repair. Until recently, NAD⁺ was primarily considered to be an intracellular molecule (iNAD⁺), however, its extracellular species (eNAD⁺) has recently been discovered and has since been associated with a multitude of pathological conditions. Therefore, accurate quantification of eNAD⁺ in bodily fluids such as plasma is paramount to answer important research questions. In order to create a clinically meaningful and reliable quantitation method, we analyzed the relationship of cell lysis, routine clinical laboratory parameters, blood collection techniques, and pre-analytical pr

SUBMITTER: Saqr AA 

PROVIDER: S-EPMC10800990 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature