Single-cell sequencing reveals an important role of SPP1 and microglial activation in age-related macular degeneration.

ABSTRACT:

Purpose

To investigate the role of senescence-related cytokines (SRCs) in the pathophysiology of age-related macular degeneration (AMD).

Design

The whole study is based on single-cell and bulk tissue transcriptomic analysis of the human neuroretinas with or without AMD. The transcriptomic data of human neuroretinas was obtained from Gene-Expression Omnibus (GEO) database.

Methods

For single-cell transcriptomic analysis, the gene expression matrix goes through quality control (QC) filtering, being normalized, scaled and integrated for downstream analysis. The further analyses were performed using Seurat R package and CellChat R package. After cell type annotation, the expression of phenotype and functional markers of microglia was investigated and cell-cell communicati

SUBMITTER: Lei S 

PROVIDER: S-EPMC10801008 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature