Development and application of recombinase polymerase amplification assay for rapid detection of Blastocystis sp.

ABSTRACT: Blastocystis sp. is a common parasite in the intestinal tract of humans and animals. The clinical diagnosis of Blastocystis sp. mainly depends on the microscopic observation of parasite, which can lead to false-negative results. An accurate and convenient diagnostic approach for Blastocystis sp. infection is crucial for effectively preventing and controlling blastocystosis. Herein, we developed a recombinase polymerase amplification (RPA) method for detecting Blastocystis sp. The results showed that the DNA amplification by RPA established in this study could be performed within 5 min at 37°C, with maximum band intensity observed at 30 min. The minimum detection limit of RPA was 100 fg μL⁻¹, consistent with conventional polymerase chain reactio

SUBMITTER: Mei X 

PROVIDER: S-EPMC10801367 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature