Rapid Generation of Murine Bispecific Antibodies Using FAST-Ig^TM for Preclinical Screening of HER2/CD3 T-Cell Engagers.

ABSTRACT: Bispecific antibodies (BsAbs) can bind to two different antigens, enabling therapeutic concepts that cannot be achieved with monoclonal antibodies. Immuno-competent mice are essential for validating drug discovery concepts, necessitating the development of surrogate mouse BsAbs. In this study, we explored the potential of FAST-Ig^TM, a previously reported BsAb technology, for mouse BsAb production. We investigated charge-based orthogonal Fab mutations to facilitate the correct assembly of heavy and light chains of mouse antibodies and employed knobs-into-holes mutations to facilitate the heterodimerization of heavy chains. We combined five anti-CD3 and two anti-HER2 antibodies in mouse IgG1 and IgG2a subclasses. These 20 BsAbs were analyzed using mass spectrometry or ion exchange

SUBMITTER: Koga H 

PROVIDER: S-EPMC10801562 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature