Minimal requirements for the epigenetic inheritance of engineered silent chromatin domains.

ABSTRACT: Mechanisms enabling genetically identical cells to differentially regulate gene expression are complex and central to organismal development and evolution. While gene silencing pathways involving DNA sequence-specific recruitment of histone-modifying enzymes are prevalent in nature, examples of sequence-independent heritable gene silencing are scarce. Studies of the fission yeast Schizosaccharomyces pombe indicate that sequence-independent propagation of heterochromatin can occur but requires numerous multisubunit protein complexes and their diverse activities. Such complexity has so far precluded a coherent articulation of the minimal requirements for heritable gene silencing by conventional in vitro reconstitution approaches. Here, we take an unconventional approach to defining th

SUBMITTER: Yuan AH 

PROVIDER: S-EPMC10801849 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature