PD-1 signaling uncovers a pathogenic subset of T cells in inflammatory arthritis.

ABSTRACT:

Background

PD-1 is an immune checkpoint on T cells, and interventions to block this receptor result in T cell activation and enhanced immune response to tumors and pathogens. Reciprocally, despite a decade of research, approaches to treat autoimmunity with PD-1 agonists have only had limited successful. To resolve this, new methods must be developed to augment PD-1 function beyond engaging the receptor.

Methods

We conducted a flow cytometry analysis of T cells isolated from the peripheral blood and synovial fluid of patients with rheumatoid arthritis. In addition, we performed a genome-wide CRISPR/Cas9 screen to identify genes associated with PD-1 signaling. We further analyzed genes involved in PD-1 signaling using publicly available bulk and single-cell RNA sequencing data

SUBMITTER: Straube J 

PROVIDER: S-EPMC10801937 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature