Paxillin participates in the sphingosylphosphorylcholine-induced abnormal contraction of vascular smooth muscle by regulating Rho-kinase activation.

ABSTRACT:

Background

The Ca²⁺-independent contraction of vascular smooth muscle is a leading cause of cardiovascular and cerebrovascular spasms. In the previous study, we demonstrated the involvement of Src family protein tyrosine kinase Fyn and Rho-kinase in the sphingosylphosphorylcholine (SPC)-induced abnormal and Ca²⁺-independent contraction of vascular smooth muscle, but the specific mechanism has not been completely clarified.

Methods

Paxillin knockdown human coronary artery smooth muscle cells (CASMCs) and smooth muscle-specific paxillin knockout mice were generated by using paxillin shRNA and the tamoxifen-inducible Cre-LoxP system, respectively. CASMCs contraction was observed by time-lapse recording. The vessel contractility was measured by using a myog

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10801962 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature