MethScore as a new comprehensive DNA methylation-based value refining the prognosis in acute myeloid leukemia.

ABSTRACT:

Background

Changes in DNA methylation are common events in the pathogenesis of acute myeloid leukemia (AML) and have been repeatedly reported as associated with prognosis. However, studies integrating these numerous and potentially prognostically relevant DNA methylation changes are lacking. Therefore, we aimed for an overall evaluation of these epigenetic aberrations to provide a comprehensive NGS-based approach of DNA methylation assessment for AML prognostication.

Results

We designed a sequencing panel targeting 239 regions (approx. 573 kb of total size) described in the literature as having a prognostic impact or being associated with AML pathogenesis. Diagnostic whole-blood DNA samples of adult AML patients divided into a training (n = 128) and a testing cohort (n = 50)

SUBMITTER: Sestakova S 

PROVIDER: S-EPMC10802002 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature