Development of molecular identification methods for Dryophytes suweonensis and D. japonicus, and their hybrids.

ABSTRACT:

Background

As hybridization can reduce biodiversity or cause extinction, it is important to identify both purebred parental species and their hybrids prior to conserving them. The Suwon tree frog, Dryophytes suweonensis, is an endangered wildlife species in Korea that shares its habitat and often hybridizes with the Japanese tree frog, D. japonicus. In particular, D. suweonensis, D. japonicus, and their hybrids often have abnormal ovaries and gonads, which are known causes that could threaten their existence.

Methods

We collected 57 individuals from six localities where D. suweonensis is known to be present. High-resolution melting curve (HRM) analysis of the mitochondrial 12S ribosomal RNA gene was performed to determine the maternal speci

SUBMITTER: Yoo N 

PROVIDER: S-EPMC10802155 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature