Loss of essential outer membrane functions causes drug hypersensitization in <i>Acinetobacter baumannii</i> overexpressing multidrug efflux pumps.

ABSTRACT: Elevated expression of Resistance-Nodulation-Cell Division (RND) drug transporters is commonly observed in clinical isolates of Acinetobacter baumannii, a nosocomial pathogen associated with multidrug-resistant (MDR) infections. We describe here a CRISPRi platform directed toward identifying essential gene hypomorphs that preferentially change resistance to the fluoroquinolone antibiotic ciprofloxacin in RND pump overproducers. An sgRNA library including single and double nucleotide mutations directed against essential genes of A. baumannii was constructed and introduced into multiple strain backgrounds, allowing strain-specific, titratable knockdown efficiencies to be analyzed. Other than NusG depletions, there were few candidates in the absence of drug treatment that showed

SUBMITTER: Hamami E 

PROVIDER: S-EPMC10802289 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature