Cholesterol-Dependent Membrane Deformation by Metastable Viral Capsids Facilitates Entry.

ABSTRACT: Non-enveloped viruses employ unique entry mechanisms to breach and infect host cells. Understanding these mechanisms is crucial for developing antiviral strategies. Prevailing perspective suggests that non-enveloped viruses release membrane lytic peptides to breach host membranes. However, the precise involvement of the viral capsid in this entry remains elusive. Our study presents direct observations elucidating the dynamically distinctive steps through which metastable reovirus capsids disrupt host lipid membranes as they uncoat into partially hydrophobic intermediate particles. Using both live cells and model membrane systems, our key finding is that reovirus capsids actively deform and permeabilize lipid membranes in a cholesterol-dependent process. Unlike membrane lytic peptides, thes

SUBMITTER: Jiao M 

PROVIDER: S-EPMC10802578 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature