Secreted Particle Information Transfer (SPIT) - A Cellular Platform for In Vivo Genetic Engineering.

ABSTRACT: A multitude of tools now exist that allow us to precisely manipulate the human genome in a myriad of different ways. However, successful delivery of these tools to the cells of human patients remains a major barrier to their clinical implementation. Here we introduce a new cellular approach for in vivo genetic engineering, Secreted Particle Information Transfer (SPIT) that utilizes human cells as delivery vectors for in vivo genetic engineering. We demonstrate the application of SPIT for cell-cell delivery of Cre recombinase and CRISPR-Cas9 enzymes, we show that genetic logic can be incorporated into SPIT and present the first demonstration of human cells as a delivery platform for in vivo genetic engineering in immunocompetent mice. We succ

SUBMITTER: Charlesworth CT 

PROVIDER: S-EPMC10802600 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature