A mini-review on gene delivery technique using nanoparticles-mediated photoporation induced by nanosecond pulsed laser.

ABSTRACT: Nanosecond pulsed laser induced photoporation has gained increasing attention from scholars as an effective method for delivering the membrane-impermeable extracellular materials into living cells. Compared with femtosecond laser, nanosecond laser has the advantage of high throughput and low costs. It also has a higher delivery efficiency than continuous wave laser. Here, we provide an extensive overview of current status of nanosecond pulsed laser induced photoporation, covering the photoporation mechanism as well as various factors that impact the delivery efficiency of photoporation. Additionally, we discuss various techniques for achieving photoporation, such as direct photoporation, nanoparticles-mediated photoporation and plasmonic substrates mediated photoporation. Among these techn

SUBMITTER: Du X 

PROVIDER: S-EPMC10802807 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature