Photoblueing of organic dyes can cause artifacts in super-resolution microscopy.

ABSTRACT: Illumination of fluorophores can induce a loss of the ability to fluoresce, known as photobleaching. Interestingly, some fluorophores photoconvert to a blue-shifted fluorescent molecule as an intermediate on the photobleaching pathway, which can complicate multicolor fluorescence imaging, especially under the intense laser irradiation used in super-resolution fluorescence imaging. Here, we discuss the mechanisms of photoblueing of fluorophores and its impact on fluorescence imaging, and show how it can be prevented.

SUBMITTER: Helmerich DA 

PROVIDER: S-EPMC10802917 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature