The CellRaft AIR^Ⓡ system: A novel system enabling organoid imaging, identification, and isolation.

ABSTRACT: Three-dimensional (3D) culture systems have been developed that can re-capitulate organ level responses, simulate compound diffusion through complex structures, and assess cellular heterogeneity of tissues, making them attractive models for advanced in vitro research and discovery. Organoids are a unique subtype of 3D cell culture that are grown from stem cells, are self-organizing, and closely replicate in vivo pathophysiology. Organoids have been used to understand tissue development, model diseases, test drug sensitivity and toxicity, and advance regenerative medicine. However, traditional organoid culture methods are inadequate because they are low throughput and ill-suited for single organoid imaging, phenotypic assessment, and isolation from heterogenous organoid populations. To addr

SUBMITTER: Stern A 

PROVIDER: S-EPMC10802934 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature