Eliminating the invading extracellular and intracellular FnBp⁺ bacteria from respiratory epithelial cells by autophagy mediated through FnBp-Fn-Integrin α5β1 axis.

ABSTRACT:

Background

We previously found that the respiratory epithelial cells could eliminate the invaded group A streptococcus (GAS) through autophagy induced by binding a fibronectin (Fn) binding protein (FnBp) expressed on the surface of GAS to plasma protein Fn and its receptor integrin α5β1 of epithelial cells. Is autophagy initiated by FnBp⁺ bacteria via FnBp-Fn-Integrin α5β1 axis a common event in respiratory epithelial cells?

Methods

We chose Staphylococcus aureus (S. aureus/S. a) and Listeria monocytogenes (L. monocytogenes/L. m) as representatives of extracellular and intracellular FnBp⁺ bacteria, respectively. The FnBp of them was purified and the protein function was confirmed by western blot, viable bacteria count,

SUBMITTER: Meng M 

PROVIDER: S-EPMC10803403 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature