Effects of guanidinoacetic acid on in vitro rumen fermentation and microflora structure and predicted gene function.

ABSTRACT: The fermentation substrate was supplemented with 0% guanidinoacetic acid (GAA) (control group, CON), 0.2% GAA (GAA02), 0.4% GAA (GAA04), 0.6% GAA (GAA06) and 0.8% GAA (GAA08) for 48 h of in vitro fermentation. Gas production was recorded at 2, 4, 6, 8, 12, 24, 36, and 48 h of fermentation. The gas was collected, and the proportions (%, v/v) of H₂, CH₄ and CO₂ were determined. The rumen fermentation parameters, including pH, ammonia nitrogen (NH₃-N), microbial protein (MCP) and volatile fatty acids (VFAs), were also determined. Furthermore, the bacterial community structure was analyzed through 16S rRNA high-throughput sequencing. The gene functions were predicted using PICRUSt1 according to the Kyoto Encyclopedia of Genes and Genomes (KEGG

SUBMITTER: Dong C 

PROVIDER: S-EPMC10803542 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature