Stromal localization of inactive CD8⁺ T cells in metastatic mismatch repair deficient colorectal cancer.

ABSTRACT:

Background

The determinants of metastasis in mismatch repair deficiency with high levels of microsatellite instability (MSI-H) in colorectal cancer (CRC) are poorly understood. Here, we hypothesized that distinct immune and stromal microenvironments in primary tumors may discriminate between non-metastatic MSI-H CRC and metastatic MSI-H CRC.

Methods

We profiled 46,727 single cells using high-plex imaging mass cytometry and analyzed both differential cell type abundance, and spatial distribution of fibroblasts and immune cells in primary CRC tumors with or without metastatic capacity. We validated our findings in a second independent cohort using immunohistochemistry.

Results

High-plex imaging mass cytometry and hierarchical clustering based on microenvironmental marke

SUBMITTER: Kucukkose E 

PROVIDER: S-EPMC10803761 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature