A transposon-based genetic marker for conspecific identity within the Bactrocera dorsalis species complex.

ABSTRACT: Here we describe a molecular approach to assess conspecific identity that relies on the comparison of an evolved mutated transposable element sequence and its genomic insertion site in individuals from closely related species. This was explored with the IFP2 piggyBac transposon, originally discovered in Trichoplusia ni as a 2472 bp functional element, that was subsequently found as mutated elements in seven species within the Bactrocera dorsalis species complex. In a B. dorsalis [Hendel] strain collected in Kahuku, Hawaii, a degenerate 2420 bp piggyBac sequence (pBac^Bd-Kah) having ~ 94.5% sequence identity to IFP2 was isolated, and it was reasoned that common species, or strains within species, should share the same evolved element and its precise genomic insertion site. To test

SUBMITTER: Zimowska GJ 

PROVIDER: S-EPMC10803768 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature