L-arginine metabolism inhibits arthritis and inflammatory bone loss.

ABSTRACT:

Objectives

To investigate the effect of the L-arginine metabolism on arthritis and inflammation-mediated bone loss.

Methods

L-arginine was applied to three arthritis models (collagen-induced arthritis, serum-induced arthritis and human TNF transgenic mice). Inflammation was assessed clinically and histologically, while bone changes were quantified by μCT and histomorphometry. In vitro, effects of L-arginine on osteoclast differentiation were analysed by RNA-seq and mass spectrometry (MS). Seahorse, Single Cell ENergetIc metabolism by profilIng Translation inHibition and transmission electron microscopy were used for detecting metabolic changes in osteoclasts. Moreover, arginine-associated metabolites were measured in the serum of rheumatoid arthritis (RA) and pre-RA patients

SUBMITTER: Cao S 

PROVIDER: S-EPMC10803985 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature