Broad Spectrum Enantioselective Amide Bond Synthetase from Streptoalloteichus hindustanus.

ABSTRACT: The synthesis of amide bonds is one of the most frequently performed reactions in pharmaceutical synthesis, but the requirement for stoichiometric quantities of coupling agents and activated substrates in established methods has prompted interest in biocatalytic alternatives. Amide Bond Synthetases (ABSs) actively catalyze both the ATP-dependent adenylation of carboxylic acid substrates and their subsequent amidation using an amine nucleophile, both within the active site of the enzyme, enabling the use of only a small excess of the amine partner. We have assessed the ability of an ABS from Streptoalloteichus hindustanus (ShABS) to couple a range of carboxylic acid substrates and amines to form amine products. ShABS displayed superior activity to a previously studied ABS, McbA, and

SUBMITTER: Tang Q 

PROVIDER: S-EPMC10804368 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature