Coupling High-Throughput and Targeted Screening for Identification of Nonobvious Metabolic Engineering Targets.

ABSTRACT: Identification of metabolic engineering targets is a fundamental challenge in strain development programs. While high-throughput (HTP) genetic engineering methodologies capable of generating vast diversity are being developed at a rapid rate, a majority of industrially interesting molecules cannot be screened at sufficient throughput to leverage these techniques. We propose a workflow that couples HTP screening of common precursors (e.g., amino acids) that can be screened either directly or by artificial biosensors, with low-throughput targeted validation of the molecule of interest to uncover nonintuitive beneficial metabolic engineering targets and combinations hereof. Using this workflow, we identified several nonobvious novel targets for improving p-coumaric acid (p-CA) a

SUBMITTER: Babaei M 

PROVIDER: S-EPMC10804409 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature