Active vitamin D increases myogenic differentiation in C2C12 cells via a vitamin D response element on the myogenin promoter.

ABSTRACT: Background: Skeletal muscle development during embryogenesis depends on proliferation of myoblasts followed by differentiation into myotubes/multinucleated myofibers. Vitamin D (VD) has been shown to affect these processes, but there is conflicting evidence within the current literature on the exact nature of these effects due to a lack of time course data. With 20%-40% of pregnant women worldwide being VD deficient, it is crucial that a clearer understanding of the impact of VD on myogenesis is gained. Methods: A detailed 8-day differentiation time course was used where C2C12 cells were differentiated in control media (2% horse serum) or with different concentrations of active VD, 1,25 (OH)₂D₃ (10^-13 M, 10^-11 M, 10^-9 M o

SUBMITTER: Alliband KH 

PROVIDER: S-EPMC10804454 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature