New application of bimetallic Ag/Pt nanoplates in a colorimetric biosensor for specific detection of E. coli in water.

ABSTRACT: A fast and sensitive aptasensor was developed using nanoplates with peroxidase activity as a novel approach. E. coli detection is described using a silver/platinum nanoplate (Ag/Pt NPL) that interacts with an oligonucleotide aptamer as a bioreceptor. The size of the Ag/Pt NPLs was about 42 nm according to the FE-SEM images. The EDS result indicates that a thin layer of Pt ions was coated on the surface of the Ag NPLs. This nanobiosensor has the ability to specifically bind to E. coli, increasing the peroxidase activity of the apt-Ag/Pt NPL. Finally, the blue color of the solution in the contaminated water samples was increased in the presence of 3,3',5,5'-tetramethylbenzidine (TMB) as a substrate and H₂O₂. The assay can be completed in 30 min and the pre

SUBMITTER: Pebdeni AB 

PROVIDER: S-EPMC10804531 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature